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Prostate cancer-specific lysine 53 acetylation of cytochrome c drives metabolic reprogramming and protects from apoptosis in intact cells

Reporting summary Further information on research design is available in the Nature Portfolio Reporting Summary linked to this Article

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ABSTRACT Vitamin B12 deficiency can present with variable hematological, neuropsychiatric, and mucocutaneous changes

To obtain the Retromer trimer, the GST-tagged Vps29 and Vps35 co-expressed cell pellet was mixed with His-Vps26A and resuspended in lysis buffer containing 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 2 mM 2-Mercaptoethanol, 50 g/ml benzamidine and DNase I before being processed through a Constant System TS-Series cell disruptor

Furthermore, dialkylamino-coumarin is a class of dye known for their low quantum yields