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Front Microbiol (2015) 6:21

In response, ROS/RNS can have harmful effects on cellular components, including mitochondria, which further promotes the generation of free radicals and increases intracellular oxidative stress

Intracellular SAM, the one carbon-derived methyl donor, is synthesized by methionine and ATP in the presence of methionine adenosine transferase (Figure 1)

Transfection, BSO treatment, and exposure of AQ-QI and DEAQ-QI were identical as described in section Transient Transfection of Human GSTP1 Gene and Cell Viability Assay. After exposure the medium was gently removed and the later steps were conducted according to manufacturers manual

Furthermore, increased mitochondrial H 2 O 2 production in MIA PaCa-2 and PANC1 cells following BPM31510 treatment was sufficient to disrupt the cellular redox balance as evidenced by increased oxidation of three distinct cellular redox probes (DCFDA, CellRox Green, and CellRox Red) (Fig

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