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After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice
Middle East respiratory syndrome coronavirus in bats, Saudi Arabia
10.1038/nn.2736 Nat Neurosci
Mustafa, M.A
Moreover, individuals with LC had higher levels of antibodies to SARS-CoV-2, EBV and VZV antigens
