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The upper layer (400 L) was transferred to an ultrafiltration tube (Ultra-free MC PLHCC, HMT, centrifugal filter unit 5 kDa) and was centrifuged (9,100 g , 4C, 120 min), followed by ultrafiltration

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doi: 10.3390/molecules26237175 139

Liu H, Mu L, Tang J et al (2014a) A potential wound healing-promoting peptide from frog skin

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