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Lower doses reach lower steady states, which may be sufficient for some goals while inadequate for others
The differentiation protocol utilized was developed and optimized from the published literatures 18,19,20,21 , specifically chosen for its capacity to generate iHLOs with high CYP enzyme activity, particularly CYP3A4, which is crucial for drug metabolism and toxicology evaluations
This systematic approach ensures research quality, enables meaningful comparison with published literature, and contributes to the evidence base that may eventually support regulatory evaluation of BPC-157 for potential therapeutic applications
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