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20 mM Tris-HCl pH 7.4 and 1 mM EDTA) supplemented with 1 cOmplete mini EDTA-free protease inhibitor cocktail (Sigma-Aldrich), incubated on ice for 10 min, lysed using 20 passes through a syringe affixed to a 23G needle and centrifuged at 1,000 g for 10 min

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Clemente-Surez VJ, Beltrn-Velasco AI, Redondo-Flrez L, Martn-Rodrguez A, Tornero-Aguilera JF

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