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Varying amounts of monobromobimane stock solution (0-300 l) were added to respective cuvettes already containing 5 l of the enzyme solution (protein concentration of 810 g ml -1 ), glutathione (100 l of stock solution) and phosphate buffer (3.0 ml)

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First, a special amino acid in the enzymes active siteoften selenocysteine in key isoformsgets oxidized by the peroxide, forming an intermediate called selenenic acid

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[DOI] [PMC free article] [PubMed] [Google Scholar] 76.Kimur I., Tanizaki Y., Sato S., Saito K., Takahashi K

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