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Depending on the study design, DSIP may serve as a comparator or probe for investigating how specific peptide signals relate to circadian patterns, stress responses, and downstream molecular readouts

After the ethanol was removed the next day, the cells were re-suspended in ice-cold PBS, treated with RNAse A (Keygen Biotech, China) at 37 C for 30 min, and then incubated with the DNA staining solution propidium iodide (PI, Keygen Biotech, China) at 4 C for 30 min

Wang, J.D

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Pri-miRNA can contain up to six miRNA precursors (pre-miRNA), which are formed by cleavage of pri-miRNA by RNase III in the presence of nuclear proteins DGCR8 (DiGeorge Syndrome Critical Region 8) and Drosha