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Par-4-dependent apoptosis by the dietary compound Withaferin A in prostate cancer cells

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GGT activity was calculated by obtaining the slope of the linear range of each sample (Abs per min) and dividing by the slope of a 4-nitroaniline standard curve (Abs per nmoles pNA) and by the protein mass (mg) to determine the reaction rate of the sample (nmoles pNA min 1 mg 1 )

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Interestingly, no metabolic pathways appeared to show significantly different activity between the gdh3 / gdh3 mutant and the wild-type strain when grown in arginine medium (Fig

2023) supports this hypothesis