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More recently, the phenomenon of G protein-mediated endosomal signaling has been appreciated, with studies demonstrating such signaling by the PTH [107], V2 vasopressin [57, 108] and 2 ARs [109]

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In all our previous murine studies we have used short cycles (1014 days) of very low subcutaneal doses (20 g/kg/day) after carefully assessing its circulating concentrations and using the minimum amount that was sufficient to adequately restore physiological values of the molecule