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6 Discussion and perspectives Entering the twenty-first century, humankind has encountered unprecedented challenges such as a century of change, the epidemic of the century, regional conflicts, and natural disasters, as well as the complex and serious global food security situation

Disulfide bonds were reduced with 10 mM dithiothreitol for 45 min at 56 C

In the study by Tandan and collaborators, 4 g/day of l-threonine were given for 6 months to patients with amyotrophic lateral sclerosis (Tandan et al 1996)

doi: 10.1016/j.freeradbiomed.2007.02.015

doi: 10.3389/fncel.2016.00084 127 LaSalleJ

19: 64350 Besheer A, Hause G, Kressler J, et al