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Resuspended samples were loaded onto equilibrated spin columns (2000 g , 2 min), passing the entire sample twice through the column

Nature 512 , 324327 (2014)

Caudle WM (2011) Using omics to define pathogenesis and biomarkers of Parkinsons disease

Co-expression affinity purification assay to identify Gid subunits binding Gid12 Baculoviruses that express individual Gid subunits (Strep-Gid1, Gid2, Gid4 Gid5, Gid7, Gid8, Gid9, or Moh1), or combinations of subunits (baculovirus AB1 co-expressing GST-Gid1, Strep-Gid5 and Gid12, AB3 co-expressing GST-Gid1, Gid5 and Gid12, BC2 co-expressing Gid7, Strep-Gid8 and Gid9, BC4 co-expressing Gid7, Gid8 and Gid9and CD4 co-expressing Gid2, Gid4 and Moh1) were mixed in various combinations to test effects of loss of various subunits on interactions with Gid12 (Fig

To learn more about how GHK-Cu works at the molecular level, see our complete GHK-Cu research guide
