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These may include: Muscle cramps or weakness Increased heart rate Seizures in individuals with seizure disorders Worsening of hypothyroid symptoms Gastrointestinal discomfort in sensitive individuals If any of these symptoms occur, its important to discontinue use and seek medical advice immediately

Funding This study was supported by the National Natural Science Foundation of China (81904224), the Peak Academic Talent Project of Jiangsu Province Hospital of Chinese Medicine (y2021rc02), and the Jiangsu Provincial Medical Key Discipline (Laboratory) Cultivation Unit (js2252)

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Furthermore, it has been shown that 2472 hour zero-calorie fasting causes liver mitochondrial oxidative stress in rats by increasing mitochondrial free radical leak and reactive oxygen species (ROS) generation at complex III level in vivo studies [45, 46]