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Verification of successful transformation and sample integrity were determined by DNA sequencing

Real-time [Ca 2+ ] cyt imaging in the entire plant Transgenic Arabidopsis plants stably expressing GCaMP3 were imaged with a motorized epifluorescence (SZX10, Olympus) stereomicroscope equipped with a 1 objective lens and a C13440 digital camera (ORCA-Flash4.0 V3, Hamamatsu Photonics)