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Using carbon-13 tracing experiments, we demonstrate that fatty acids and glucose are prominent sources of acetyl-CoA that can feed acetyl-CoA pools and histone acetylation in the absence of ACLY and ACSS2

doi: 10.1016/j.genhosppsych.2025.12.010 Asta Ratna Prajapati , Sion Scott , Alexandra L Dima , Allan Clark , Jo Taylor , Jonathan Wilson , Debi Bhattacharya

Immunofluorescence and microscopy Frozen brain tissue sections (8 m thickness) in glass slides were washed with PBS, fixed in acetone-methanol (11 v/v) fixative, blocked the cellular antigen with 3% bovine serum albumin at room temperature for 1 hr in the presence of 0.4% Triton X-100 and incubated with respective primary antibodies such as mouse anti-cPT1/ant-cPT2 (1250 dilution) and rabbit anti-neurofilament (1250 dilution) for overnight at 4C

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