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PMID: 39482401 J Biol Chem A technique for delineating the unfolding requirements for substrate entry into retrotranslocons during endoplasmic reticulum-associated degradation

IKE has been described as a potent system (SLC7A11 inhibitor and ferroptosis inducer in several tumor models, primarily through depletion of intracellular cysteine/glutathione and accumulation of lipid peroxides [16] 19

J., Brach, T., Marty, L., Kreye, S., Rouhier, N., Jacquot, J

We have previously shown that the cell death associated with lysosomotropic compounds can be rescued by Bafilomycin A, which increases lysosomal pH and prevents compound accumulation, further supporting lysosomal accumulation as the cause for cell death [54]

Prepare your work area: sanitize the surface with isopropyl alcohol, wash hands, and put on gloves

Which volume to use depends on the pharmacological question e.g
