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The cells were lysed in lysis buffer [10 mM Tris (pH 7.4), 10 mM KCl, 2 mM MgCl, 1 mM EGTA, 1 mM DTT, 0.05% (v/v) Triton X-100 and 1 protease inhibitor (Pierce)] for 30 min on ice

Its DNMT inhibitor effect was first reported in breast cancer cells, where it induced global DNA demethylation and restored the activation of certain TSGs

Figure 4B shows the elevated activity of ALDH2 FA-metabolizing enzymes in mouse brains

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