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Methods Cell culture A549, MiaPaCa2, DAN-G, SW1990, MPanc96, SUIT2-013-NQO1+/, HPAFII, ASPC1, PL45, H596, Hs766T, BxPC3, MBA-MD-231+/, and MCF7 cell lines were obtained from ATCC, tested for mycoplasma contamination and grown in complete Dulbecco's Modified Eagle's Medium (DMEM) with 10 % fetal bovine serum (FBS)

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The progression of HT is also impacted by epigenetic modifications like miRNA levels, oxidative stress

Sorting was performed using a 100 m nozzle with a pressure of 20 PSI

This combination of features makes GHK-Cu a promising candidate for supporting tissue repair and remodeling, protecting against oxidative stress, and enhancing cellular communication