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Water retention (mild, typically 2-4 pounds) occurs in about 30% of users during the first 2-3 weeks

Rev Endocr Metab Disord (2018) 19:293300

J Immunol 143:197481, PMID: 2789253 Markovic J, Borrs C, Ortega A, Sastre J, Via J, Pallard FV (2007) Glutathione is recruited into the nucleus in early phases of cell proliferation

doi: 10.3390/biomedicines9020156

Practical limits depend on sterile techniquemost research protocols target fewer than 30 punctures per vial
