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The worms were lysed using a pre-chilled stainless steel homogenizer (Wheaton, 357572) with 15 plunger strokes and the protein concentration of the lysate was determined using a Pierce BCA protein assay kit (Thermo Scientific, 23227)

Search parameters and filtering criteria included a precursor mass tolerance of 10 ppm, a fragment ion tolerance of 0.6 Da, and an allowance of up to three missed cleavages

Ascorbate chemistry: antioxidant and other functions The chemistry of ascorbate is surprisingly complex and has been well-reviewed [6], [7], [8]

Dehydration or lack of nutrients can also make it worse
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