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Endogenous co-immunoprecipitation LnCaP cells were lysed and scraped in cold lysis buffer (20 mM Tris-HCl, Ph 8, 130 mM NaCl, 1% Triton X-100, 1 mM DTT, 10 mM NaF, 1 complete EDTA-free protease cocktail tablet (Roche), Phosphatase cocktail inhibitors II and IV (Sigma))

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BCR cross-linking led to activation of NOTCH1 signaling, which was significantly higher in CLL/N M cells compared to CLL/N WT , as evidenced by western blot analysis (Fig

494 This disruption results in a less stable knee joint at the moment of ground contact, increasing the risk of valgus collapse or anterior tibial translation, which are common mechanisms of ACL injury

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