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Description
Equal amounts of cellular total proteins (20 g) were separated using SDS-PAGE and then transferred to polyvinylidene difluoride membranes (Millipore, MA, USA)

Czeisler CA, Duffy JF, Shanahan TL, et al
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IGFBP-3 proteolysis has also been described in insulin resistant states such as fasting, obesity and type 1 and 2 diabetes (62, 124, 125)
