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At 15 min post-ligation, venograms were taken, captured, and digitized into files on a personal computer and were analyzed using ISSA image software (Vamstec, Zagreb, Croatia)

With saline medication initiated at 15 min ligation-time, controls at 1 min thereafter showed scant karyopyknosis in cerebral cortex, with increasing number of karyopyknotic neurons at 5 min thereafter

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