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doi: 10.3390/antiox9060521

Glutathione is a tripeptide molecule composed of three amino acids: L-glutamine, L-cysteine, and L-glycine

Bead pools were resuspended in BSS (500 mM Tris-HCl pH 7.5, 1.5 M NaCl, 50 g/L BSA) and incubated in absence or presence of 10 nM TCEs at 37 C for varying amounts of time, as reported in the figures (typically 24, 8, 4, 2, 1, 0.5, 0.25 h)

doi:10.3390/healthcare11131946 2

Without adequate selenium, glutathione becomes trapped in its oxidized form, reducing antioxidant capacity [17]

These siRNA oligos were transfected into cells at 50% confluence for 6 h using Lipofectamine 3000 Kit (L3000015, Thermo Fisher Scientific, USA), following the manufacturers instructions