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Ratios of classical monocytes (CD14-/CD16-), which are more responsible for phagocytosis and initiation/priming of the immune system [254], are higher in patients with ALS and pre-symptomatic SOD1 carriers as compared with healthy controls, but function at a decreased efficacy and invade the CNS, though the difference between the roles of monocytes in the CNS versus systemic circulation are still unknown [255]

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The Q-Exactive + - Orbitrap-MS, equipped with a HESI probe, was set up with the following parameters: a heat temperature of 425 C, sheath gas at 30, auxiliary gas at 13, sweep gas at 3, a spray voltage of 3.5 kV in positive mode, a capillary temperature of 320 C, and an S-lens setting of 45

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