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Surprisingly, deletion of PNPLA2 does not result in obesity, likely due to counter-regulatory mechanisms such as reductions in food intake and reduced PPAR signaling in adipose tissue (239)

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Short answer: one missed day changes nothing measurable

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Animals were euthanatized by placement in a carbon dioxide chamber at 6 or 18 h post-ICA-1s administration, after which they sustained cervical dislocation as a secondary means of euthanasia

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