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Note, portal hypertension, along with caval and intracranial hypertension and aortal hypotension, was regularly eliminated/attenuated [129,130,131,132,133,134,135,136,137,138,139,140,141,142]

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And the 96-well plates were incubated for another 3 h at 37 C and followed by determining the absorbance at 450 nm utilizing a microplate reader (Thermo, USA)

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