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In case the study is on humans, the sample size must be calculated to achieve a statistical power of at least 80% [473]

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Some factors that influence how long the shot lasts include: Lifestyle and stress levels Diet and alcohol consumption Existing health conditions (e.g., pernicious anemia, digestive disorders) How quickly your body uses or excretes B12 If your energy starts fading or symptoms return before your next scheduled shot, let your provider know