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The 25-hydroxyvitamin D 3 1--hydroxylase enzyme is encoded by the CYP27B1 (cytochrome P450 27B1) gene

ROS are generated by the NADPH oxidase (NOX) enzymes, located on the plasma membrane of cells, notably in neutrophils and vascular endothelial cells

GCH1/BH4/DHFR system A recent genome-wide CRISPR activation screen has identified GTP-cyclic hydrolase 1 (GCH1), a potent antioxidant that scavenges free radicals (RTA), as the key enzyme responsible for tetrahydrobiopterin (BH4) synthesis

and iii) a mono or polyglutamate chain of variable length (Fig

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