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Cells were washed with FACS buffer followed by fixation with the Foxp3 fixation/permeabilization kit (eBioscience) in accordance with the manufacturer's instructions, and stained with intracellular antibodies for 60 min at 4 C

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For example, VitB12 possesses scavenging activity, and thus, its absence may directly contribute to an increase in oxidative stress

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Mechanisms such as DNA damage, mitochondrial dysfunction, and telomere shortening further accelerate lung aging and compromise respiratory health [93]