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Briefly, the full-length or truncated EBOV L (residues 11400) with an N-terminal 2Strep-His 6 -MBP-Flag tag were co-expressed with the full-length or truncated eVP35 (residues 80340) with an N-terminal His 6 -MBP tag

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This minimizes waste across the entire protocol

[DOI] [PubMed] [Google Scholar] 136.Gamulin O., Oroz K., Coric L., Krajacic M., Skrabic M., Dretar V., Strbe S., Talapko J., Juzbasic M., Krezic I., et al