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Glucose-6-phosphate dehydrogenase (G6PD) activity assay The activity of G6PD was determined using the G6PD Assay Kit (Sigma-Aldrich) according to the manufacturers instructions

[DOI] [PMC free article] [PubMed] [Google Scholar] Zahnd C

To assess the effects of kinase inhibitors, cells were treated simultaneously with 5 M GSK-3 inhibitor SB216763 (Tocris), 5 M CDK5 inhibitor Roscovitine (Tocris), or 5 M calpain inhibitor PD150606 (Tocris), each combined with 5 M BSA-PC for 24h

For SDS-PAGE, 5-20 g of total protein was used and semi-dry blotted on PVDF membranes

Of note are the preferential distributions of the transsulfuration pathway and the catabolizing enzyme glycine N-methyltransferase in mammalian tissues such as liver and kidney (Finkelstein, 1990)