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During an organisms metabolism, ROS are continuously generated in various organelles and through multiple metabolic pathways [118]

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In vitro succinylation assay Purified GCLC proteins were incubated with different concentrations of succinyl-CoA (S1129, Sigma-Aldrich) in TBS buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl) at 37 C for 4 h

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