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In contrast, VEGFR2 levels remained unchanged in Rab27a knockdown sprouts and were marginally elevated in immunoblots of endothelial cells (Fig
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For F-actin quantification, at the end of experiment, cells were trypsinized then fixed with PBS/ formalin 3% and incubated with PBS/ Triton 0.1%/ TRITC-phallodin 1 g/mL (Sigma Aldrich) during 5 min

Gallelli et al

J Hazard Mater 223224:112