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How long does the procedure take

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The difference between a clean preparation and a ruined one often comes down to three or four small habits that take seconds to follow

doi: 10.1007/s42000-018-0033-5 105 ChartoumpekisDVZirosPGHabeosIGSykiotisGP

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The supernatant was transferred into a 96-well plate and incubated with the assay cocktail containing MES-buffer, co-factor and enzyme mixture, and Ellman's reagent for 30 min
