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The adrenal glands also possess an independent circadian oscillator: oscillatory Clock/BMAL1, Per1-3 and Cry1 expression is seen in the outer adrenal cortex (zona glomerulosa and zona fasciculata)

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Immunofluorescence assay SK-MEL-147 cells were collected and seeded into the inner 60 wells of 96-well, black-walled plates (Agilent, 204626-100) at 1000 cells per well in 100 uL of complete DMEM overnight in a 37 C incubator with 5% CO 2

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