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As vitamin B12 seems to be better absorbed on an empty stomach (or better yet, acidic environment), you should try to wait for a few hours after having your evening meal before taking them

Two types of medications for ulcers and gastric reflux proton-pump inhibitors, such as omeprazole (Prilosec) and lansoprazole (Prevacid), and H2 blockers like cimetidine (Tagamet), famotidine (Pepcid), and ranitidine (Zantac) inhibit the release of stomach acid necessary to free dietary vitamin B12

Even though vitamin B12 shots are most effective in those who have a deficiency in the vitamin, it is hard to know if you have a deficiency and most people will be able to experience the positive effects of regular vitamin B12 injections

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But some people need to take vitamin B12 supplements to prevent or treat a deficiency

To ensure the absorbance from oil red O staining was within the linear detection range of the plate reader, a calibration curve was established for oil red O staining in adipocytes using a previously described protocol.[23] 2.12