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One piece of liver was fixed in 4% paraformaldehyde for liver histology and the remainder frozen in liquid nitrogen for biochemical and gene-protein expression analyses

The 5-ASA used will depend on the location and disease type

Sequencing was performed on an Illumina NextSeq 550 instrument in readmode SR75 by the Next Generation Sequencing facility at Vienna BioCenter Core Facilities (VBCF)

In particular, Cu 2+ ions play a pivotal role in modulating A aggregation

RPTEC were maintained in Renal Epithelial Cell Basal Medium (REBM, ATCC) supplemented with 0.5% FBS, 10 nM triiodothyronine, 10 ng ml 1 rh EGF, 100 ng ml 1 hydrocortisone hemisuccinate, 5 g ml 1 rh insulin, 1 M epinephrine, 5 g ml 1 transferrin and 2.4 mM L-alanyl-L-glutamine

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