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Several problems can lead to a vitamin B12 or folate deficiency

Samples were processed as follows: the proteins were precipitated with acetone/TCA (Sigma-Aldrich)

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To effectively deliver CD38 inhibitors to target cells, a series of biological barriers must be overcome, such as cell membranes and intracellular barriers

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Children's requirements are lower and increase with age: infants aged 0-6 months have a safe intake of about 0.3 g daily, those aged 712 months need 0.4 g daily, children aged 13 years require 0.5 g, those aged 46 years need 0.8 g, and children aged 710 years should receive 1.0 g daily