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Fragment ions at m/z of 92.1 and 77.9 were additionally used for the detection and confirmation of 1-MNA, respectively

This remarkable selectivity stems from 5-amino-1MQs specific interaction with the nicotinamide-binding pocket of NNMT, rather than the SAM-binding site common to many methyltransferases

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While a balanced diet or oral supplements are often sufficient for maintaining adequate B12 levels, certain conditions may necessitate vitamin B12 injections

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According to StatPearls (NCBI Bookshelf), the most common adverse effect of intramuscular vitamin B12 injections is significant pain at the injection site, which is particularly noted in thinner individuals