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The cell suspension was sonicated using mechanical ultrasonicator (4 s intervals of 50 Hz repeated 10 times) followed by 3 cycles of freezing of cell lysate in liquid N 2 and thawing at room temperature
It attenuates reactive oxygen species accumulation, protecting immune cells like macrophages and lymphocytes from dysfunction
The fragment pJRD215-tac was obtained from plasmid pJRD215-tac- cup after digestion with Bam H I and Eco R I
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Anderson, C., Milne, G
The above experiments suggest that the interaction between luteolin and GPVI may have influenced the binding of GPVI to collagen
