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The synthesis conditions used were as follows: an initial cycle at 50 C for 2 min, one cycle of polymerase activation at 95 C for 10 min, followed by 40 cycles with a denaturation temperature at 95 C for 15 s, an alignment of 60 C by 60 s and an extension at 72 C by 45 s

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Considered the increased risk of morbidity and mortality if a second cardiac surgery was performed, percutaneous closure is the preferred treatment choice