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doi: 10.1016/j.metabol.2021.154886 118 SkjaerbaekCFrystykJOrskovHKissmeyer-NielsenPJensenMBLaurbergSet al

Metabolite set enrichment analysis (MSEA) was conducted to identify metabolic pathways associated with the observed metabolite patterns (Fig

Save a small aliquot from each step of the purification for analysis by SDS-PAGE to monitor the location of the fusion protein throughout the purification (see Notes 13 and 14)

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While in some cases the ghrelin peptide is not required for these modifications to occur, in others, the presence is necessary for these changes to take effect