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The team monitored the activity of PACAP-producing neurons using GCaMP6, a calcium indicator that fluoresces in response to elevated intracellular calcium, providing a proxy for neuronal firing

Swenson et al., 1994

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Lentivirusmediated knockdown using a scramble (scr) or Pfkfb3specific shRNA in proliferating NPCs in vitro (Fig 7C) reduced lactate production (Fig 7D), but did not significantly alter total mitochondrial oxygen consumption rate (OCR) as a readout of oxidative phosphorylation (Fig 7E) or OCR dedicated to ATP production (Fig EV5H)
