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doi: 10.1016/j.alcohol.2008.12.009

The cells were washed twice with PBS, 400 l of PBS were added to each well, and fluorescence intensity was determined with a flow cytometer (analyzed by FlowJo 7.6.1)

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It is co-secreted with insulin in a molar ratio that generally stays the same but which may be modified by disease states, including diabetes and obesity