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Separation of soluble and insoluble components Single mouse hippocampal tissue was isolated, and 300 l mixed protein lysis solution with RIPA (Applygen, C1053), a protease inhibitor (Merck, 539134), and a phosphatase inhibitor (Merck, 524628) (=100:1:2) were added

The pudendal nerve, the levator ani nerve, and branches from the S2 S4 nerve roots innervate the pelvic floor muscles

Brain-derived neurotrophic factor (BDNF) is required for the enhancement of hippocampal neurogenesis following environmental enrichment

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3-Isobutyl-1-methylxanthine (IBMX), poly(D-lysine), and forskolin were from Sigma-Aldrich