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Quantitative real-time polymerase chain reaction (PCR) analysis Total RNA was extracted using a TRIzol kit (Invitrogen, Carlsbad, CA, USA) and cDNA synthesis was performed using a cDNA synthesis kit (Fermentas Life Sciences) according to the manufacturer instructions

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Untreated cells were included to serve as a baseline control
Apoptosis, proliferation, and sex hormone receptors in superficial parts of human endometrium at the end of the secretory phase

these symptoms usually improve over time without treatment (Zakko 2018)

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