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7 L of RNA was added to the RT and buffer mixture prepared with random hexamers and oligoDT primers as per kit instructions, and rtPCR carried out with the following programme: 10 min at 25C, 30 min at 55C and 5 min at 85C

Stress: Chronic stress can exhaust your body and brain, leading to a lack of mental clarity

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Low-Impact Aerobic Conditioning Exercises that increase the heart rate and blood flowwithout jarring the spinehelp to rehabilitate and maintain the lower back
