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The discovery and characterization of a potent DPP-IV inhibitory peptide from oysters for the treatment of type 2 diabetes based on computational and experimental studies

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The purified RNCs from the in vitro translation (IVT) reaction of the His-SDD1 model mRNA using the IVT extract prepared from Ub-K48only-uS10 mutant strain ( A ): uS10ski2 expressing Ub-K48only-uS10-3HA from plasmid pST321, or Ub-K63only-uS10 mutant strain ( B ): uS10ski2 expressing Ub-K63only-uS10-3HA from plasmid pST320, were incubated with the RQT complex in the absence of ATP and then separated by sucrose density gradient centrifugation