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Label-Free Visual Imaging The above lipid labeling methods based on staining and fluorescence imaging are often interfered by the intrinsic properties and normal function of biomolecules and may suffer from the physical and chemical environment in C

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Nuclei were isolated from frozen, ground plant tissue using ice-cold Honda buffer (0.44 M sucrose, 1.25% (w/v) Ficoll, 2.5% (w/v) dextran T40, 20 mM HEPES-KOH pH 7.4, 10 mM MgCl 2 , 0.5% (w/v) Triton X-100, 1 mM DTT, 1 protease inhibitor (Roche) and 0.4 U l 1 Ribolock RNase inhibitor (Thermo Fisher Scientific)) and collected via centrifugation at 2,000 g for 5 min at 4 C